Cardiac Troponin

Cardiac troponin scorecard.

The recent opinion paper by Apple (1 ) on a cardiac troponin assay scorecard has enhanced the conundrum on whether cardiac troponin assays are clinically usable or not. Apple has proposed a scorecard that evaluates cardiac troponin assays according to the total imprecision at the 99th percentile. The cited data include the concentrations at the 10% CV and whether the value is greater or less th...

CARDIAC TROPONIN I.cdr

Cardiac Troponin Assays

Troponin: Its Utility in Acute Coronary Syndrome Case Presentation 1: J.P. is an obese 55-year-old male with a history of gastroesophageal reflux disease who presents to the emergency department with substernal chest pain that he attributes to a “large meal.” J.P. is pale and diaphoretic. An ECG shows tachycardia with normal sinus rhythm. J.P.’s troponin levels (decision limit 0.04 g/L) at pres...

Chimeric cardiac troponin I antibodies

most assay manufacturers take additional measures during the assay development. The most frequent procedure involves suppressing the non-specific binding with blocking reagents, which vary from normal mouse IgG to more refined formulations. Whilst in most cases these measures are adequate, they do result in additional costs in terms of both material and labor. The effect of HAMA can also be sup...

Hydrodynamic properties of bovine cardiac troponin-I and troponin-T.

Bovine cardiac troponin-I (TN-I) and troponin-T (TN-T) have been examined in solution using ultracentrifugation, gel filtration, and viscosity. A new method of purifying TN-T, employing hydroxylapatite chromatography in 6 M urea, is reported. Cardiac TN-T (Mr = 36,000) undergoes a reversible, concentration-dependent association in nondenaturing buffers, probably to a tetramer. The Stokes radius...